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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial role in promoting mobile-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been explained. Two added splice variants had been claimed but thorough nucleotide sequence details is not really nevertheless accessible.
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Blocking peptides are peptides that bind particularly into the goal antibody and block antibody binding. These peptide commonly contains the epitope identified because of the antibody.
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This tutorial outlines further probable optimization of cellular and immunoassay parameters to make certain the very best success are...
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This guide outlines more attainable optimization of mobile and immunoassay parameters to make certain the best possible success are...
Antibodies certain to the blocking peptide not bind to your epitope to the target protein. By comparing the staining from the blocked antibody versus the antibody on your own, you can see which staining is specific.
Concentrate on Information Cyclin E belongs to the highly conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance throughout the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation styles which lead to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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